Acidic cultivation of Haematococcus pluvialis for improved astaxanthin production in the presence of a lethal fungus 机翻标题: 暂无翻译,请尝试点击翻译按钮。

来源
Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies
年/卷/期
2019 / 278 /
页码
138-144
ISSN号
0960-8524
作者单位
Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea;Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea;Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea;
作者
Choi, Hong Il;Sim, Sang Jun;Hwang, Sung-Won;
摘要
An acidic cultivation strategy was developed to prevent contamination of a lethal fungus Paraphysoderma sedebokerensis in Haematococcus pluvialis culture for astaxanthin production. Instead of generally used neutral pH, an acidic condition (pH 4) was applied to the cultivation, resulting in a significant inhibition of the fungal contamination. This could be ascribed to the acidity-associated denaturation of a surface protein of P. sedebokerensis, which plays an important role in recognition of H. pluvialis. Stress relief strategies including stepwise light irradiation and naturally occurring nitrogen deficiency were employed in the induction stage to minimize the reduction of astaxanthin production caused by acidic pH. Accordingly, an astaxanthin titer of 84.8 mg L-1 was obtained, which is 141-fold of that from the completely contaminated culture and double of that without the stress relief methods. This strategy provides a persistent contamination control method that can be used for practical astaxanthin production by H. pluvialis.
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关键词/主题词
Haematococcus pluvialis;Paraphysoderma sedebokerensis;Acidic cultivation;Stress relief;Astaxanthin;
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