(JP3759628) PURPOSE:To impart a plant with resistance to a herbicide inhibiting zeta-carotene desaturase of a plant as well as to a herbicide inhibiting phytoene desaturase of a plant and to enable the use of these herbicides as a marker in transformation. CONSTITUTION:A plant resistant to a herbicide for inhibiting zeta-carotene desaturase is produced by preparing a DNA sequence coding a polypeptide (phytoene desaturase) having an enzymatic activity to convert phytoene through zeta-carotene to lycopene and introducing the DNA sequence into a plant in a state to enable the manifestation of the genetic information. A marker for the confirmation of the transformation to the herbicide inhibiting zeta-carotene desaturase is composed of a DNA sequence coding a polypeptide having an enzymatic activity to convert phytoene to lycopene.
(JP3759628) 1. An enzyme which converts lycopenes phytoene genus Erwinia activity of the gene encoding phytoene desaturase, their genetic information in a state capable of expressing, Agrob.alpha method, electroporation of transformed in any way possibleperforming photosynthesis and introducing higher plants, ζ - inhibiting herbicide-resistant method for the production of the PCC. 2. The phytoene desaturase Erwinia genus, Erwinia uredovoraor Erwinia herbicoladerived from, produced according to claim 1 method. 3. The phytoene desaturase polypeptide, amino acid sequence shown in Fig. 6-7 having the amino acid sequence shown Erwinia genus polypeptide, or 1 in which one or more of the amino acid sequence of an amino acid substitution, deletion, insertion, or addition of a polypeptide having an amino acid sequence, to produce a method according to claim 2. 4. The phytoene desaturase polypeptide, a transit, the method any one of claims 1-3 produce. 5. An enzyme which converts lycopenes phytoene Erwinia activity from a gene encoding a genus phytoene desaturase, ζ - PCC inhibits the action of the active herbicide resistance to transformed plants for confirmingmarker. 6. The phytoene desaturase Erwinia genus, Erwinia uredovora or Erwinia herbicoladerived from, according to claim 5 marker. 7. The phytoene desaturase polypeptide, amino acid sequence shown in Fig. 6-7 having the amino acid sequence shown Erwinia genus polypeptide, or 1 in which one or more of the amino acid sequences of an amino acid substitution, deletion, insertion, or addition of a polypeptide having an amino acid sequence, according to claim 6 marker.
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