Mutant RpsA gene and method for producing L-amino acid 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US2013005000 A1 2013-01-03 [US20130005000]US8975045 B2 2015-03-10 [US8975045] / 2013-01-032015-03-10
申请号/申请日
2012US-13569264 / 2012-08-08
发明人
DOI HIDETAKA;HAGIWARA YUSUKE;USUDA YOSHIHIRO;
申请人
AJINOMOTO;
主分类号
IPC分类号
C07K-014/24C07K-014/245C12N-001/21C12N-015/31C12P-013/04C12P-013/08
摘要
(US8975045) A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided.  A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at position 210 is replaced with another amino acid residue is used.  This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.
机翻摘要
暂无翻译结果,您可以尝试点击头部的翻译按钮。
地址
代理人
代理机构
;
优先权号
2010JP-0025000 2010-02-08 2011WO-JP52469 2011-02-07
主权利要求
(US8975045) The invention claimed is: 1.  A method for producing an L-amino acid, the method comprising: A) culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability in a medium containing a carbon source selected from the group consisting of a fatty acid and an alcohol; and   B) collecting the L-amino acid from the medium,   wherein the bacterium expresses an ribosomal protein S1 RpsA protein comprising the amino acid sequence of SEQ ID NO: 2, wherein said RpsA protein has a mutation in which the aspartic acid residue at position 210 of SEQ ID NO: 2 is replaced with a different amino acid residue, and wherein said bacterium is Escherichia coli. 2. The method according to claim 1, wherein the different amino acid residue is a tyrosine residue. 3. The method according to claim 1, wherein the bacterium does not express an RpsA protein not having said mutation. 4. The method according to claim 1, wherein the chromosome of the bacterium expresses a mutant rpsA gene coding for the RpsA protein having the mutation. 5. The method according to claim 1, wherein the carbon source is a fatty acid. 6. The method according to claim 5, wherein the fatty acid is oleic acid. 7. The method according to claim 5, wherein the fatty acid is a mixture of fatty acids derived from fat or oil. 8. The method according to claim 1, wherein the carbon source is an alcohol. 9. The method according to claim 8, wherein the alcohol is glycerol. 10. The method according to claim 8, wherein the alcohol is ethanol. 11. The method according to claim 1, wherein the carbon source is a mixture of a fatty acid and glycerol obtained by hydrolyzing fat or oil.
法律状态
(US8975045) LEGAL DETAILS FOR US2013005000  Actual or expected expiration date=2031-05-31    Legal state=ALIVE    Status=GRANTED     Event publication date=2012-08-08  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US13569264  Application date=2012-08-08  Standardized application number=2012US-13569264     Event publication date=2012-09-19  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment OWNER: AJINOMOTO CO., INC., JAPAN ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:DOI, HIDETAKA HAGIWARA, YUSUKE USUDA, YOSHIHIRO SIGNING DATES FROM 20120906 TO 20120907 REEL/FRAME:028986/0968     Event publication date=2013-01-03  Event code=US/A1  Event type=Examination events  Application published  Publication country=US  Publication number=US2013005000  Publication stage Code=A1  Publication date=2013-01-03  Standardized publication number=US20130005000     Event publication date=2015-03-10  Event code=US/B2  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as second publication  Publication country=US  Publication number=US8975045  Publication stage Code=B2  Publication date=2015-03-10  Standardized publication number=US8975045     Event publication date=2015-03-10  Event code=US/354  Event indicator=Pos  Event type=Event indicating In Force  Event type=Extension of term of duration of protection  Patent term extension under  35 U.S.C 154(b) until/for:  Number of days of extension=113
专利类型码
A1B2
国别省市代码
若您需要申请原文,请登录。

最新评论

暂无评论。

登录后可以发表评论

意见反馈
返回顶部