(US8975045) A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided. A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at position 210 is replaced with another amino acid residue is used. This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.
(US8975045) The invention claimed is: 1. A method for producing an L-amino acid, the method comprising: A) culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability in a medium containing a carbon source selected from the group consisting of a fatty acid and an alcohol; and B) collecting the L-amino acid from the medium, wherein the bacterium expresses an ribosomal protein S1 RpsA protein comprising the amino acid sequence of SEQ ID NO: 2, wherein said RpsA protein has a mutation in which the aspartic acid residue at position 210 of SEQ ID NO: 2 is replaced with a different amino acid residue, and wherein said bacterium is Escherichia coli. 2. The method according to claim 1, wherein the different amino acid residue is a tyrosine residue. 3. The method according to claim 1, wherein the bacterium does not express an RpsA protein not having said mutation. 4. The method according to claim 1, wherein the chromosome of the bacterium expresses a mutant rpsA gene coding for the RpsA protein having the mutation. 5. The method according to claim 1, wherein the carbon source is a fatty acid. 6. The method according to claim 5, wherein the fatty acid is oleic acid. 7. The method according to claim 5, wherein the fatty acid is a mixture of fatty acids derived from fat or oil. 8. The method according to claim 1, wherein the carbon source is an alcohol. 9. The method according to claim 8, wherein the alcohol is glycerol. 10. The method according to claim 8, wherein the alcohol is ethanol. 11. The method according to claim 1, wherein the carbon source is a mixture of a fatty acid and glycerol obtained by hydrolyzing fat or oil.
(US8975045) LEGAL DETAILS FOR US2013005000 Actual or expected expiration date=2031-05-31 Legal state=ALIVE Status=GRANTED Event publication date=2012-08-08 Event code=US/APP Event indicator=Pos Event type=Examination events Application details Application country=US US13569264 Application date=2012-08-08 Standardized application number=2012US-13569264 Event publication date=2012-09-19 Event code=US/AS Event type=Change of name or address Event type=Reassignment Assignment OWNER: AJINOMOTO CO., INC., JAPAN ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNORS:DOI, HIDETAKA HAGIWARA, YUSUKE USUDA, YOSHIHIRO SIGNING DATES FROM 20120906 TO 20120907 REEL/FRAME:028986/0968 Event publication date=2013-01-03 Event code=US/A1 Event type=Examination events Application published Publication country=US Publication number=US2013005000 Publication stage Code=A1 Publication date=2013-01-03 Standardized publication number=US20130005000 Event publication date=2015-03-10 Event code=US/B2 Event indicator=Pos Event type=Event indicating In Force Granted patent as second publication Publication country=US Publication number=US8975045 Publication stage Code=B2 Publication date=2015-03-10 Standardized publication number=US8975045 Event publication date=2015-03-10 Event code=US/354 Event indicator=Pos Event type=Event indicating In Force Event type=Extension of term of duration of protection Patent term extension under 35 U.S.C 154(b) until/for: Number of days of extension=113
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