Gliocladium roseum strains useful for the control of fungal pathogens in plants 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
US6495133 B1 2002-12-17 [US6495133] / 2002-12-17
申请号/申请日
1999US-09407285 / 1999-09-29
发明人
XUE ALLEN G;
申请人
CANADA DEPARTMENT OF AGRICULTURE & AGRI- FOOD;
主分类号
IPC分类号
A01N-063/04C12N-001/14
摘要
(US6495133) A biocontrol agent and a method of use thereof for controlling diseases caused by fungal pathogens in plants.  In one aspect of the invention, novel strains of Gliocladium roseum exhibiting antagonistic effects against plant pathogens are used as biocontrol agents.  Most preferably, a novel strain Gliocladium roseum ACM941 (ATCC #74447) is used in a treatment effective against fungal pathogens of plants.  The biocontrol agent of the present invention may be used in the treatment of seeds, soil or plants to effectively protect against plant diseases caused by fungal pathogens.  The biocontrol agent is particularly effective against fungal infections of pea, bean, canola, wheat, barley, beet, broccoli, brussel sprouts, cabbage, canola, califlower, cucumber, egg plant, pepper, tomato, marigold and other horticultural and ornamental plants, caused by Alternaria alternata, Aphanomyces euteiches, Ascochyta spp., Bipolaris sorokiniana, Fusarium graminearum, Fusarium oxysporum f.sp. pisi, Fusarium solani f.sp. pisi, Fusarium spp.  Mycosphaerella pinodes, Pythium spp., Rhizopus sp., Rhizoctonia solani, and Sclerotinia sclerotiorum.
机翻摘要
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地址
代理人
代理机构
;
优先权号
1998US-60102582 1998-09-30 1999US-09407285 1999-09-29
主权利要求
(US6495133) What is claimed is: 1.  A biologically pure culture of a strain of a microorganism Gliocladium roseum exhibiting antagonistic effects against a plant pathogen, said microorganism having the identifying characteristics of ATCC -74447. 2. The culture of claim 1, having antagonistic effects against at least one pathogen selected from the groups of Ascomycetes, Deuteromycetes, Oomycetes and Zygomycetes. 3. The culture of claim 1, wherein the microorganism is capable of protection against fungal pathogens causing seed decay, foot/root rots, seedling blight, head blight and wilt diseases in plants of Asteraceae, Brassicaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Poaceae and Solanaceae. 4. The culture of claim 1, wherein the microorganism is capable of protection against are soil-borne and seed-borne Rhizoctonia solani on pea, bean, beet, canola, broccoli, brussel sprouts, cabbage, cauliflower, cucumber, egg plant, pepper, tomato and marigold, soil-borne and seed-borne Alternaria alternata, Aphanomyces euteiches, Ascochyta spp., Fusarium oxysporum, Fusarium solani, Mycosphaerella pinodes, Pythium aphanidermatum, Pythium ultimum, Rhizopus sp., and Sclerotinia sclerotiorum on pea, and soil-borne and seed-borne Bipolaris sorokiniana and Fusarium graminearum on wheat and barley. 5. A composition comprising: a culture of a strain of Gliocladium roseum exhibiting antagonistic effects against a plant pathogen, said strain of Gliocladium roseum having the identifying characteristics of ATCC -74447;  and a delivery medium. 6. The composition of claim 5, wherein the delivery medium is a plant seed. 7. The composition according to claim 6, wherein the seed is chosen from the group consisting of seeds of wheat, seeds of barley, seeds of canola, seeds of sugar beet, seeds of table beet, seeds of dry bean, seeds of garden bean, seeds of field pea, seeds of sweet pea, seeds of broccoli, seeds of brussel sprouts, seeds of cabbage, seeds of cauliflower, seeds of cucumber, seeds of egg plant, seeds of pepper, seeds of tomato, and seeds of marigold. 8. The composition according to claim 5, including at least one fungicide. 9. The composition according to claim 8, wherein the at least one fungicide is at least one fungicide intended for use with field crops, horticultural crops, vegetables or ornamentals. 10. A method of protecting a plant from fungal infection comprising the steps of contacting the plant during a stage of the growth of said plant or contacting a seed of said plant with a strain of a microorganism Gliocladium roseum exhibiting antagonistic effects against a fungal plant pathogen, said strain having the identifying characteristics of ATCC -74447. 11. The method of claim 10, wherein the seed of said plant is immersed into a composition comprising said strain before said seed is planted in a growth medium for said plant and said plant is grown. 12. The method according claim 10 wherein said plant comprises plant seedlings or seeds and said plant is planted in a growth medium containing said strain. 13. A method of protecting pea plants against PRRC pathogens, said method comprising: isolating a culture of a strain of a microorganism Gliocladium roseum exhibiting antagonistic effects against fungal plant pathogens, said strain having the identifying characteristics of ATCC -74447;  treating a seed with said strain of said microorganism;  and planting said seed in a soil environment. 14. A biologically pure culture of a strain of a microorganism Gliocladium roseum exhibiting antagonistic effects against a plant pathogen, characterized by bands of 1.3 kb following DNA amplification with UBC519 primer and of 0.5 kb and 1.3 kb following DNA amplification with UBC521 primer. 15. The culture of claim 14, having antagonistic effects against at least one pathogen selected from the groups of Ascomycetes, Deuteromycetes, Oomycetes and Zygomycetes. 16. The culture of claim 14, wherein the microorganism is capable of conferring protection against fungal pathogens causing seed decay, foot/root rots, seedling blight, head blight and wilt diseases in plants of Asteraceae, Brassicaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Poaceae and Solanaceae. 17. The culture of claim 14, wherein the microorganism is capable of conferring protection against are soil-borne and seed-borne Rhizoctonia solani on pea, bean, beet, canola, broccoli, brussel sprouts, cabbage, cauliflower, cucumber, egg plant, pepper, tomato and marigold, soil-borne and seed-borne Alternaria alternate, Aphanomyces euteiches, Ascochyta spp., Fusarium oxysporum, Fusarium solani, Mycosphaerella pinodes, Pythium aphanidermatum, Pythium ultimum, Rhizopus sp., and Sclerotinia sclerotiorum on pea, and soil-borne and seed-borne Bipolaris sorokiniana and Fusarium graminearum on wheat and barley. 18. A composition comprising: a culture of a strain of Gliocladium roseum exhibiting antagonistic effects against a plant pathogen, characterized by bands of 1.3 kb following DNA amplification with UBC519 primer and of 0.5 kb and 1.3 kb following DNA amplification with UBC521 primer;  and a delivery medium. 19. The composition of claim 18, wherein the delivery medium is a plant seed. 20. The composition according to claim 19, wherein the seed is chosen from the group consisting of seeds of wheat, seeds of barley, seeds of canola, seeds of sugar beet, seeds of table beet, seeds of dry bean, seeds of garden bean, seeds of field pea, seeds of sweet pea, seeds of broccoli, seeds of brussel sprouts, seeds of cabbage, seeds of cauliflower, seeds of cucumber, seeds of egg plant, seeds of pepper, seeds of tomato, and seeds of marigold. 21. The composition according to claim 18 including at least one fungicide. 22. The composition according to claim 18, wherein the at least one fungicide is at least one fungicide intended for use with field crops, horticultural crops, vegetables or ornamentals. 23. A method of protecting a plant from fungal infection comprising contacting a plant during a stage of the growth of said plant with a strain of a microorganism Gliocladium roseum exhibiting antagonistic effects against a fungal plant pathogen, characterized by bands of 1.3 kb following DNA amplification with UBC519 primer and of 0.5 kb and 1.3 kb following DNA amplification with UBC521 primer. 24. The method of claim 23, wherein a seed of said plant is immersed into a composition comprising said strain before said seed is planted in a growth medium for said plant and said plant is grown. 25. The method according to claim 23 wherein said plant comprises plant seedlings or seeds and said plant is planted in a growth medium containing said strain. 26. A method of protecting pea plants against PRRC pathogens, said method comprising: isolating a culture of a strain of a microorganism Gliocladium roseum exhibiting antagonistic effects against fungal plant pathogens characterized by bands of 1.3 kb following DNA amplification with UBC519 primer and of 0.5 kb and 1.3 kb following DNA amplification with UBC521 primer;  treating a seed with said strain of said microorganism;  and planting said seed in a soil environment.
法律状态
(US6495133) LEGAL DETAILS FOR US6495133  Actual or expected expiration date=2019-09-29    Legal state=ALIVE    Status=GRANTED     Event publication date=1999-09-29  Event code=US/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=US US09407285  Application date=1999-09-29  Standardized application number=1999US-09407285     Event publication date=1999-09-29  Event code=US/EXMR  Event type=Administrative notifications  USPTO Examiner Name Primary Examiner: WITZ, JEAN C    Event publication date=1999-09-29  Event code=US/ART  Event type=Administrative notifications  USPTO Art Group  ART=1651     Event publication date=1999-09-29  Event code=US/ENT  Event type=Administrative notifications  Entity Status Set to Undiscounted Business Entity Status: UNDISCOUNTED    Event publication date=1999-09-29  Event code=US/AIA  Event type=Administrative notifications  First Inventor File Indicated:  AIA=No     Event publication date=1999-09-29  Event code=US/DK  Event type=Examination events  Attorney Docket Number Docket Nbr: 41603-A    Event publication date=1999-09-29  Event code=US/CUST  Event type=Examination events  Attorney/Agent Customer Number Customer Nbr: 23529    Event publication date=1999-09-29  Event code=US/354  Event indicator=Pos  Event type=Extension of term of duration of protection  Patent term extended under  35 U.S.C 154(b) until/for Delays (A,B,C): 0  Overlap Delays: 0  Non Overlap Delays: 0   PTO Office Delays: 0  Applicant Delays: 0  Adjustment total:  Number of days of extension=0    Event publication date=1999-12-22  Event code=US/DOCK  Event indicator=Pos  Event type=Examination events  Case Docketed to Examiner    Event publication date=2000-01-12  Event code=US/APE  Event type=Corrections  Preliminary amendments    Event publication date=2000-01-13  Event code=US/AS  Event type=Change of name or address  Event type=Reassignment  Assignment Owner: HER MAJESTY THE QUEEN IN RIGHT OF CANADA AS REPRES  Effective date of the event=1999-11-15  ASSIGNMENT OF ASSIGNORS INTEREST ASSIGNOR:XUE, ALLEN G. REEL/FRAME:010503/0359     Event publication date=2000-01-24  Event code=US/IDS  Event type=Examination events  Event type=OAI  Information Disclosure Statement Filed    Event publication date=2001-03-26  Event code=US/CTNF  Event type=Examination events  Event type=OA  Event type=OAO  Non-Final Rejection    Event publication date=2001-07-05  Event code=US/POAC  Event type=Change of name or address  Change in Power of Attorney (May Include Associate POA)    Event publication date=2001-10-09  Event code=US/AINEA  Event type=OAI  Event type=Examination events  Informal or Non-Responsive Amendment after Examiner Action    Event publication date=2001-11-13  Event code=US/CTNFR  Event type=Examination events  Event type=OAI  Response after Non-Final Action    Event publication date=2002-02-20  Event code=US/SUPRSP  Event type=OAI  Supplemental Response    Event publication date=2002-07-10  Event code=US/NOAM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Mail Notice of Allowance    Event publication date=2002-10-24  Event code=US/APRDY  Event indicator=Pos  Event type=Examination events  Application Is Considered Ready for Issue    Event publication date=2002-11-28  Event code=US/PAT  Event indicator=Pos  Event type=Event indicating In Force  Patented Case    Event publication date=2002-11-28  Event code=US/ISSM  Event indicator=Pos  Event type=Examination events  Event type=OAO  Event type=Restitution or restoration  Issue Notification Mailed    Event publication date=2002-12-17  Event code=US/B1  Event indicator=Pos  Event type=Event indicating In Force  Granted patent as first publication  Publication country=US  Publication number=US6495133  Publication stage Code=B1  Publication date=2002-12-17  Standardized publication number=US6495133     Event publication date=2005-12-27  Event code=US/NMFP  Event type=Payment or non-payment notifications  Publication of First Notice of Maintenance Fees Payable. 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专利类型码
B1
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