(EP2650376) In a method for producing an L-amino acid comprising culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium, a bacterium which has been subjected to a modification selected from the group consisting of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.
(EP2650376) 1. A method for producing an L-amino acid comprising culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium, wherein the method comprises reducing intracellular hydrogen peroxide concentration of the bacterium. 2. The method according to claim 1, wherein the bacterium has been modified so that the intracellular hydrogen peroxide concentration is reduced. 3. The method according to claim 1 or 2, wherein a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium. 4. The method according to any one of claims 1 to 3, wherein the bacterium belongs to the genus Escherichia, Pantoea, or Enterobacter. 5. The method according to claim 4, wherein the bacterium is Escherichia coli, Pantoea ananatis, or Enterobacter aerogenes. 6. The method according to any one of claims 2 to 5, wherein the bacterium has been subjected to a modification selected from the group consisting of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof. 7. The method according to claim 6, wherein the oxyS gene encodes RNA having the nucleotide sequence of SEQ ID NO: 9, or a conservative variant thereof. 8. The method according to claim 6 or 7, wherein the fixABC genes encode proteins having the amino acid sequences of SEQ ID NOS: 11, 13, and 15, or a conservative variant thereof. 9. The method according to any one of claims 3 to 8, wherein the substance that reduces the intracellular hydrogen peroxide concentration is thiourea. 10. The method according to any one of claims 1 to 9, wherein the carbon source is a fatty acid. 11. The method according to claim 10, wherein the fatty acid is oleic acid. 12. The method according to claim 10, wherein the fatty acid is a mixture of fatty acids derived from a fat or oil. 13. The method according to any one of claims 1 to 9, wherein the carbon source is an alcohol. 14. The method according to claim 13, wherein the alcohol is glycerol. 15. The method according to claim 13, wherein the alcohol is ethanol. 16. The method according to any one of claims 1 to 9, wherein the carbon source is a mixture of a fatty acid and glycerol obtained by hydrolyzing a fat or oil. 17. The method according to claim 15, wherein the bacterium is Escherichia coli, and has been modified so that it can aerobically utilize ethanol. 18. The method according to any one of claims 1 to 17, wherein the L-amino acid is L-lysine. 19. The method according to claim 18, wherein activity or activities of one or more kinds of enzymes selected from the group consisting of dihydrodipicolinate reductase, diaminopimelate decarboxylase, diaminopimelate dehydrogenase, phosphoenolpyruvate carboxylase, aspartate aminotransferase, diaminopimelate epimerase, aspartate semialdehyde dehydrogenase, tetrahydrodipicolinate succinylase, and succinyldiaminopimelate deacylase are enhanced, and/or activity of lysine decarboxylase is attenuated.
(EP2650376) LEGAL DETAILS FOR EP2650376 Actual or expected expiration date=2031-12-08 Legal state=ALIVE Status=PENDING Event publication date=2011-12-08 Event code=EP/APP Event indicator=Pos Event type=Examination events Application details Application country=EP EP11846714 Application date=2011-12-08 Standardized application number=2011EP-0846714 Event publication date=2013-10-16 Event code=EP/A1 Event type=Examination events Application published with search report Publication country=EP Publication number=EP2650376 Publication stage Code=A1 Publication date=2013-10-16 Standardized publication number=EP2650376 Event publication date=2013-10-16 Event code=EP/17P Event indicator=Pos Event type=Examination events Request for examination filed Pruefungsantrag gestellt Effective date of the event=2013-07-08 Event publication date=2013-10-16 Event code=EP/AK Event indicator=Pos Event type=Designated states Designated contracting states: Benannte vertragsstaaten AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Event publication date=2014-03-19 Event code=EP/DAX Event indicator=Neg Event type=Designated states Request for extension of the european patent (to any country) deleted Event publication date=2016-07-20 Event code=EP/A4 Event indicator=Pos Event type=Examination events Supplementary search report Ergaenzender recherchenbericht Publication country=EP Publication number=EP2650376 Publication stage Code=A4 Publication date=2016-07-20 Standardized publication number=EP2650376 Event publication date=2016-07-20 Event code=EP/RA4 Event indicator=Pos Event type=Administrative notifications Date and kind of supplementary search report (correction) Despatch of supplementary search report Effective date of the event=2016-06-21 Event publication date=2016-07-20 Event code=EP/RIC1 Event type=Classification modifications Classification (correction) Klassifikation (korr.) IPC: C07K 14/245 20060101ALI20160613BHEP Event publication date=2016-07-20 Event code=EP/RIC1 Event type=Classification modifications Classification (correction) Klassifikation (korr.) IPC: C12N 15/09 20060101ALI20160613BHEP Event publication date=2016-07-20 Event code=EP/RIC1 Event type=Classification modifications Classification (correction) Klassifikation (korr.) IPC: C12N 1/38 20060101ALI20160613BHEP Event publication date=2016-07-20 Event code=EP/RIC1 Event type=Classification modifications Classification (correction) Klassifikation (korr.) IPC: C12N 1/20 20060101ALI20160613BHEP Event publication date=2016-07-20 Event code=EP/RIC1 Event type=Classification modifications Classification (correction) Klassifikation (korr.) IPC: C12P 13/08 20060101AFI20160613BHEP Event publication date=2017-03-22 Event code=EP/17Q Event indicator=Pos Event type=Examination events First examination report Erster pruefungsbescheid Effective date of the event=2017-02-20
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