Method for producing l-amino acid 机翻标题: 暂无翻译,请尝试点击翻译按钮。

公开号/公开日
EP2650376 A4 2016-07-20 [EP2650376]EP2650376 A1 2013-10-16 [EP2650376] / 2016-07-202013-10-16
申请号/申请日
2011EP-0846714 / 2011-12-08
发明人
DOI HIDETAKA;HOSHINO YASUSHI;MASUMITSU YURI;USUDA YOSHIHIRO;
申请人
AJINOMOTO;
主分类号
IPC分类号
C07K-014/245C12N-001/20C12N-001/38C12N-015/09C12P-013/08
摘要
(EP2650376) In a method for producing an L-amino acid comprising culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium, a bacterium which has been subjected to a modification selected from the group consisting of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.
机翻摘要
暂无翻译结果,您可以尝试点击头部的翻译按钮。
地址
代理人
代理机构
;
优先权号
2011WO-JP78380 2011-12-08 JP2010276062 2010-12-10
主权利要求
(EP2650376) 1. A method for producing an L-amino acid comprising culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium,  wherein the method comprises reducing intracellular hydrogen peroxide concentration of the bacterium. 2. The method according to claim 1, wherein the bacterium has been modified so that the intracellular hydrogen peroxide concentration is reduced. 3. The method according to claim 1 or 2, wherein a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium. 4. The method according to any one of claims 1 to 3, wherein the bacterium belongs to the genus Escherichia, Pantoea, or Enterobacter. 5. The method according to claim 4, wherein the bacterium is Escherichia coli, Pantoea ananatis, or Enterobacter aerogenes. 6. The method according to any one of claims 2 to 5, wherein the bacterium has been subjected to a modification selected from the group consisting of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof. 7. The method according to claim 6, wherein the oxyS gene encodes RNA having the nucleotide sequence of SEQ ID NO: 9, or a conservative variant thereof. 8. The method according to claim 6 or 7, wherein the fixABC genes encode proteins having the amino acid sequences of SEQ ID NOS: 11, 13, and 15, or a conservative variant thereof. 9. The method according to any one of claims 3 to 8, wherein the substance that reduces the intracellular hydrogen peroxide concentration is thiourea. 10. The method according to any one of claims 1 to 9, wherein the carbon source is a fatty acid. 11. The method according to claim 10, wherein the fatty acid is oleic acid. 12. The method according to claim 10, wherein the fatty acid is a mixture of fatty acids derived from a fat or oil. 13. The method according to any one of claims 1 to 9, wherein the carbon source is an alcohol. 14. The method according to claim 13, wherein the alcohol is glycerol. 15. The method according to claim 13, wherein the alcohol is ethanol. 16. The method according to any one of claims 1 to 9, wherein the carbon source is a mixture of a fatty acid and glycerol obtained by hydrolyzing a fat or oil. 17. The method according to claim 15, wherein the bacterium is Escherichia coli, and has been modified so that it can aerobically utilize ethanol. 18. The method according to any one of claims 1 to 17, wherein the L-amino acid is L-lysine. 19. The method according to claim 18, wherein activity or activities of one or more kinds of enzymes selected from the group consisting of dihydrodipicolinate reductase, diaminopimelate decarboxylase, diaminopimelate dehydrogenase, phosphoenolpyruvate carboxylase, aspartate aminotransferase, diaminopimelate epimerase, aspartate semialdehyde dehydrogenase, tetrahydrodipicolinate succinylase, and succinyldiaminopimelate deacylase are enhanced, and/or activity of lysine decarboxylase is attenuated.
法律状态
(EP2650376) LEGAL DETAILS FOR EP2650376  Actual or expected expiration date=2031-12-08    Legal state=ALIVE    Status=PENDING     Event publication date=2011-12-08  Event code=EP/APP  Event indicator=Pos  Event type=Examination events  Application details  Application country=EP EP11846714  Application date=2011-12-08  Standardized application number=2011EP-0846714     Event publication date=2013-10-16  Event code=EP/A1  Event type=Examination events  Application published with search report  Publication country=EP  Publication number=EP2650376  Publication stage Code=A1  Publication date=2013-10-16  Standardized publication number=EP2650376     Event publication date=2013-10-16  Event code=EP/17P  Event indicator=Pos  Event type=Examination events  Request for examination filed Pruefungsantrag gestellt  Effective date of the event=2013-07-08     Event publication date=2013-10-16  Event code=EP/AK  Event indicator=Pos  Event type=Designated states  Designated contracting states: Benannte vertragsstaaten AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR    Event publication date=2014-03-19  Event code=EP/DAX  Event indicator=Neg  Event type=Designated states  Request for extension of the european patent (to any country) deleted    Event publication date=2016-07-20  Event code=EP/A4  Event indicator=Pos  Event type=Examination events  Supplementary search report Ergaenzender recherchenbericht  Publication country=EP  Publication number=EP2650376  Publication stage Code=A4  Publication date=2016-07-20  Standardized publication number=EP2650376     Event publication date=2016-07-20  Event code=EP/RA4  Event indicator=Pos  Event type=Administrative notifications  Date and kind of supplementary search report (correction) Despatch of supplementary search report  Effective date of the event=2016-06-21     Event publication date=2016-07-20  Event code=EP/RIC1  Event type=Classification modifications  Classification (correction) Klassifikation (korr.) IPC: C07K  14/245       20060101ALI20160613BHEP    Event publication date=2016-07-20  Event code=EP/RIC1  Event type=Classification modifications  Classification (correction) Klassifikation (korr.) IPC: C12N  15/09        20060101ALI20160613BHEP    Event publication date=2016-07-20  Event code=EP/RIC1  Event type=Classification modifications  Classification (correction) Klassifikation (korr.) IPC: C12N   1/38        20060101ALI20160613BHEP    Event publication date=2016-07-20  Event code=EP/RIC1  Event type=Classification modifications  Classification (correction) Klassifikation (korr.) IPC: C12N   1/20        20060101ALI20160613BHEP    Event publication date=2016-07-20  Event code=EP/RIC1  Event type=Classification modifications  Classification (correction) Klassifikation (korr.) IPC: C12P  13/08        20060101AFI20160613BHEP    Event publication date=2017-03-22  Event code=EP/17Q  Event indicator=Pos  Event type=Examination events  First examination report Erster pruefungsbescheid  Effective date of the event=2017-02-20
专利类型码
A4A1
国别省市代码
若您需要申请原文,请登录。

最新评论

暂无评论。

登录后可以发表评论

意见反馈
返回顶部