[专利]
Simple basic media for human pluripotent cell culture
申请号:
2016JP-0186896
申请日:
2016-09-26
公开号:
JP2017018137 A 2017-01-26 [JP2017018137]
公开日:
2017-01-26
申请人:
WISCONSIN ALUMNI RESEARCH FOUNDATION
专利权人:
WISCONSIN ALUMNI RESEARCH FOUNDATION
发明人:
THOMSON JAMES A;CHEN GUOKAI
国家:
(JP2017018137) PROBLEM TO BE SOLVED: To provide induction methods of pluripotent cells.SOLUTION: The method comprises a step of reprogramming human somatic cells in an albumin-free medium containing water, salts, amino acids, vitamins, glucose, insulin, FGF, selenium, transferrin, one of TGF-β, and NODAL, in an amount sufficient to support somatic cell reprogramming to derive each human iPS cell, the defined albumin-free medium not containing a component derived from non-human animals.SELECTED DRAWING: Figure 1A