An enzyme isolated from rat liver cytosol (native molecular mass 78. 3 kDa; polypeptide molecular mass 42.5 kDa) is capable of catalysing the NADH/NADPH-dependent degradation of S-nitrosoglutathione (GSNO). The activity utilizes 1 mol of coenzyme per mol of GSNO processed. The isolated enzyme has, as well, several characteristics that are unique to alcohol dehydrogenase (ADH) class III isoenzyme: it is capable of catalysing the NAD+-dependent oxidations of octanol (insensitive to inhibition by 4-methylpyrazole), methylcrotyl alcohol (stimulated by added pentanoate) and 12-hydroxydodecanoic acid, and also the NADH/NADPH-dependent reduction of octanal. Methanol and ethanol oxidation activity is minimal. The enzyme has formaldehyde dehydrogenase activity in that it is capable of catalysing the NAD+/NADP+-dependent oxidation of S-hydroxymethylglutathione. Treatment with the arginine-specific reagent phenylglyoxal prevents the pentanoate stimulation of methylcrotyl alcohol oxidation and markedly diminishes the enzymic activity towards octanol, 12-hydroxydodecanoic acid and S-hydroxymethylglutathione; the capacity to catalyse GSNO degradation is also checked. Additionally, limited peptide sequencing indicates 100% correspondence with known ADH class III isoenzyme sequences. Kinetic studies demonstrate that GSNO is an exceptionally active substrate for this enzyme. S-Nitroso-N-acetylpenicillamine and S-nitrosated human serum albumin are not substrates; the activity towards S-nitrosated glutathione mono- and di-ethyl esters is minimal. Product analysis suggests that glutathione sulphinamide is the major stable product of enzymic GSNO processing, with minor yields of GSSG and NH3; GSH, hydroxylamine, nitrite, nitrate and nitric oxide accumulations are minimal. Inclusion of GSH in the reaction mix decreases the yield of the supposed glutathione sulphinamide in favor of GSSG and hydroxylamine.
(KR20160104208) The present invention relates to a low grade quicklime composition generated from a fluidic layer boiler, and to a method for producing the same. The low grade quicklime composition has functions in removing sulfur oxide contained in sintered gas during a refining process by using a fluidic layer boiler scattering material having a large content of quicklime, improving basicity efficiency in a sintering furnace during a refining process, improving basicity efficiency during a refining process for reducing the discharging amount of carbon dioxide generated after sintering, desulfurizing sintered exhaust gas, and reducing the discharge of carbon dioxide. According to the present invention, sulfur oxide included in exhaust gas discharged as a result of a sintering process is effectively removed, and basicity efficiency in a sintering furnace is improved. Accordingly, durability of the sintering furnace is improved, and quicklime used during the sintering process is replaced, so the discharging amount of carbon dioxide generated during the sintering process is reduced. Moreover, by using the low grade quicklime composition for removing sulfur oxide according to the present invention, an effect in desulfurizing sulfur oxide in sintered exhaust gas can be easily improved without using a separate treating device.
来源期刊：Current Genetics: Eukaryotes with Emphasis on Yeasts, Fungi, Mitochondria, Plastids
年/卷/期：2019 / 65 / 2
Upstream open reading frames (uORFs) in 5 UTRs of eukaryotic mRNAs are increasingly recognized as important elements that regulate cellular protein synthesis. Since uORFs can start from non-AUG codons, an enormous number of potential uORF initiation sites exists in 5UTRs. However, only a subset of these sites is used and it has been unclear how actual start sites are selected. Studies of the DEAD-box helicase Ded1p from S. cerevisiae show that translation of uORFs with non-AUG initiation codons occurs upstream of mRNA structures that emerge with defective Ded1p. The data designate mRNA structure as important determinant for non-AUG initiation sites of uORFs. Ded1p can control this RNA structure and thereby regulate uORF translation.
A strong business growth strategy, spearheaded by James Williams, Managing Director, saw the company increase its turnover by 13.3% to ￡8.5m. By relocating its entire operations and investing in a new press, Curtis Packaging expanded its facility footprint by 70%, improved production time by 64% and optimised operational efficiencies by 20%. In acknowledgement of Curtis Packaging's 2017 successes, the company was awarded SME of the Year, UK Packaging Awards; Packaging Printer of the Year, and was highly commended for Company of the Year, PrintWeek Awards; plus, won Solutions for Packaging Development, Print Solutions Awards.
Leociley Rocha Alencar Menezes;Reinaldo Nobrega de Almeida;Renan Marinho Braga;Mairim Russo Serafini;Emmanoel Vilaca Costa;Adriano Antunes de Souza Araujo;Lucindo Jose Quintans-Junior;Paula dos Passos Menezes;Francielly de Oliveira Araujo;Tatianny Araujo Andrade;Igor Araujo Santos Trindade;Heitor Gomes de Araujo-Filho;Jullyana de Souza Siqueira Quintans
来源期刊：Current topics in medicinal chemistry
年/卷/期：2018 / 18 / 9
Background: Some research studies have shown that Lippia pedunculosa essential oil(EOLP) has interesting biological activities. However, its low water solubility is the main challenge toachieve its therapeutic potential. In this context, Cyclodextrins (CDs) have been widely used in order toovercome this problem due to your capability to improve the physicochemical properties of drugs.Objective: In this perspective, the main goal of this study was to investigate how the improvement ofthe physicochemical properties of inclusion complexes (EOLP and β-CD) enhance the antinociceptiveeffect in mice.Methods: To achieve that, we prepared samples by Physical Mixture (PM), Paste Complexation (PC)and Slurry Complexation (SC) methods, followed by their physicochemical characterization. Inaddition, it was evaluated if the use of β-CD enhances the antinociceptive effect of EOLP in mice.Results: The analysis showed that rotundifolone (72.02%) was the major compound of EOLP and wefound out based on DSC results that β-CD protected it from oxidation. In addition, TG techniquesdemonstrated that the best inclusion methods were PC and SC, due to their greater weight loss (10.8and 11.6%, respectively) in the second stage (171-312°C), indicating that more complexed oil wasreleased at the higher temperature than oil free. Other characteristics, such as changes in the typicalcrystalline form, and reduced particle size were observed by SEM and laser diffraction, respectively.The SC was the most effective complexation method, once the presence of rotundifolone was detectedby FTIR. Based on that, SC method was used in all mice tests. In this regard, the number of paw lickswas reduced for both compounds (all doses), but EOLP was more effective in reducing the nociceptivebehavior.Conclusion: Therefore, CDs seem not to be a good tool to enhance the pharmacological properties ofEOs rich in peroxide compounds such as rotundifolone.
来源期刊：International Journal of Heat and Mass Transfer
年/卷/期：2018 / 126 / Pt.A
In this study, asymmetric peristaltic nanofluid flow in a two dimensional and divergent wavy channel is modeled and the heat transfer analysis is performed for it. The modeling is performed as two-phase model and it is assumed the magnetic field effects on the nanoparticles as magnetohydrodynamic (MHD) flow. Both upper and lower channel walls are considered in constant temperature and constant nanoparticle concentration. The governing equations were solved by analytical least square method (LSM) using the Maple 15.0 mathematical software and compared to available numerical results. The effect of some parameters existed in the equations (Geometry parameter, Brownian motion parameter, Dufour (Du) and Soret (Sr) numbers and etc.), are discussed on the velocities, temperature and nanoparticles concentration functions. As an important outcome, increasing in both Dufour (Du) and Soret (Sr) numbers lead to reduction in nanoparticles concentration while Dufour number increments make larger temperature profiles in the channel. (C) 2018 Elsevier Ltd. All rights reserved.